Heptafluorobutyl chloroformate-based sample preparation protocol for nonchiral and chiral amino acid analysis by gas chromatography-mass spectrometry was written by Simek, Petr;Husek, Petr;Zahradnickova, Helena. And the article was included in Methods in Molecular Biology (New York, NY, United States) in 2019.Quality Control of Thiazolidine-4-carboxylic acid This article mentions the following:
Gas chromatog. (GC) is a commonly used technique in amino acid anal. (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives In the last two decades, alkyl chloroformates (RCFs) have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrixes, and the process can easily be coupled with liquid-liquid extraction of the resulting less polar derivatives into immiscible organic phase. Here we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate (HFBCF) followed by subsequent chiral as well as nonchiral GC/MS (mass spectrometric) anal. on a resp. nonpolar fused silica and an enantioselective Chirasil-Val capillary column. In the experiment, the researchers used many compounds, for example, Thiazolidine-4-carboxylic acid (cas: 444-27-9Quality Control of Thiazolidine-4-carboxylic acid).
Thiazolidine-4-carboxylic acid (cas: 444-27-9) belongs to thiazolidine derivatives. Thiazolidine is a heterocyclic compound with five-membered saturated ring with a thioether group and an amine group in 1 and 3 positions of the ring. Thiazolidine is prepared as it was in its first reported synthesis, by the condensation of cysteamine and formaldehyde. Other thiazolidines may be synthesized by similar condensations. A notable derivative is 4-carboxythiazolidine, derived from formaldehyde and cysteine.Quality Control of Thiazolidine-4-carboxylic acid
Referemce:
Thiazolidine – Wikipedia,
Thiazolidine – ScienceDirect.com